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Mariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae
| Resumo: | One strain of a novel genus and species of the order Planctomycetes, designated FC18T, was isolated from the epiphytic community of Fucusspiralis. This strain was non-pigmented in medium M13 but was slightly pink pigmented on medium M14, containing four-fold the levels of glucose, peptone and yeast extract of M13. The organism was primarily spherical, with unicellular non-motile forms and rosettes. The optimal temperature for growth was about 25 C and the optimal pH was 7.5. FC18T was chemoorganotrophic and aerobic. Several sugars, polyols and amino acids were assimilated. The major fatty acids were C18: 1 ω 9c, C14: 0 and C16: 0. The major polar lipids were phosphatidylglycerol (PG) and two unknown lipids. Menaquinone 5 (MK-5) was the main respiratory quinone, but MK-6 was also present. The results of the 16S rRNA gene sequence analysis confirmed the affiliation of this organism to the order Planctomycetales, family Planctomycetaceae, with Blastopirellula marina as the closest relative with only 86% sequence similarity. On the basis of physiological, biochemical and chemotaxonomic characteristics we propose that FC18T (=LMG 29748T =DSM 26290T) represents a novel species of a novel genus of the family Planctomycetaceae for which we propose the name Mariniblastusfucicola gen. nov., sp. nov. © 2017 IUMS. |
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| Assunto: | carbon source carbohydrate bacterium isolate nucleotide sequence bacterial DNA Planctomycetales farnoquinone cell structure transmission electron microscopy Portugal pH Seaweed menaquinone priority journal phylogeny classification Mariniblastus fucicola Planctomycetes Phylogeny sugar nonhuman RNA 16S DNA base composition Phosphatidylglycerols Sequence Analysis, DNA Bacterial Typing Techniques amino acid bacterium identification temperature fatty acid Rhodopirellula genetics macroalga Vitamin K 2 Article chemistry Fatty Acids sequence analysis RNA, Ribosomal, 16S peptone phosphatidylglycerol bacterial growth gene sequence glucose isolation and purification brown alga uranyl acetate DNA sequence microbiology DNA, Bacterial seaweed Rhodopirellula baltica Base Composition |
| País: | Portugal |
| Tipo de documento: | journal article |
| Tipo de acesso: | Aberto |
| Instituição associada: | Repositório Aberto da Universidade do Porto |
| Idioma: | inglês |
| Origem: | Repositório Aberto da Universidade do Porto |
| _version_ | 1850560648083144704 |
|---|---|
| conditionsOfAccess_str | open access |
| country_str | PT |
| description | One strain of a novel genus and species of the order Planctomycetes, designated FC18T, was isolated from the epiphytic community of Fucusspiralis. This strain was non-pigmented in medium M13 but was slightly pink pigmented on medium M14, containing four-fold the levels of glucose, peptone and yeast extract of M13. The organism was primarily spherical, with unicellular non-motile forms and rosettes. The optimal temperature for growth was about 25 C and the optimal pH was 7.5. FC18T was chemoorganotrophic and aerobic. Several sugars, polyols and amino acids were assimilated. The major fatty acids were C18: 1 ω 9c, C14: 0 and C16: 0. The major polar lipids were phosphatidylglycerol (PG) and two unknown lipids. Menaquinone 5 (MK-5) was the main respiratory quinone, but MK-6 was also present. The results of the 16S rRNA gene sequence analysis confirmed the affiliation of this organism to the order Planctomycetales, family Planctomycetaceae, with Blastopirellula marina as the closest relative with only 86% sequence similarity. On the basis of physiological, biochemical and chemotaxonomic characteristics we propose that FC18T (=LMG 29748T =DSM 26290T) represents a novel species of a novel genus of the family Planctomycetaceae for which we propose the name Mariniblastusfucicola gen. nov., sp. nov. © 2017 IUMS. |
| documentTypeURL_str | http://purl.org/coar/resource_type/c_6501 |
| documentType_str | journal article |
| id | 590569a4-4a84-4437-8634-41f74d2b1e3a |
| identifierHandle_str | https://hdl.handle.net/10216/120477 |
| language | eng |
| relatedInstitutions_str_mv | Repositório Aberto da Universidade do Porto |
| resourceName_str | Repositório Aberto da Universidade do Porto |
| spellingShingle | Mariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae carbon source carbohydrate bacterium isolate nucleotide sequence bacterial DNA Planctomycetales farnoquinone cell structure transmission electron microscopy Portugal pH Seaweed menaquinone priority journal phylogeny classification Mariniblastus fucicola Planctomycetes Phylogeny sugar nonhuman RNA 16S DNA base composition Phosphatidylglycerols Sequence Analysis, DNA Bacterial Typing Techniques amino acid bacterium identification temperature fatty acid Rhodopirellula genetics macroalga Vitamin K 2 Article chemistry Fatty Acids sequence analysis RNA, Ribosomal, 16S peptone phosphatidylglycerol bacterial growth gene sequence glucose isolation and purification brown alga uranyl acetate DNA sequence microbiology DNA, Bacterial seaweed Rhodopirellula baltica Base Composition |
| title | Mariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae |
| topic | carbon source carbohydrate bacterium isolate nucleotide sequence bacterial DNA Planctomycetales farnoquinone cell structure transmission electron microscopy Portugal pH Seaweed menaquinone priority journal phylogeny classification Mariniblastus fucicola Planctomycetes Phylogeny sugar nonhuman RNA 16S DNA base composition Phosphatidylglycerols Sequence Analysis, DNA Bacterial Typing Techniques amino acid bacterium identification temperature fatty acid Rhodopirellula genetics macroalga Vitamin K 2 Article chemistry Fatty Acids sequence analysis RNA, Ribosomal, 16S peptone phosphatidylglycerol bacterial growth gene sequence glucose isolation and purification brown alga uranyl acetate DNA sequence microbiology DNA, Bacterial seaweed Rhodopirellula baltica Base Composition |