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The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors

Bibliographic Details
Summary:Liposomes of the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) and the fluorinated cholesterol derivative heptafluorocholesterol (F7-CHOL) were tested at the molar ratio of 1 : 1 for DNA compaction and transfection. Their properties were correlated with the characteristics of the well-known system, DOTAP and cholesterol (CHOL). The mass lipid-DNA (L/D) ratios at the isoelectric point were within the ranges 3-4 for DOTAP: CHOL and 4-5 for DOTAP: F7-CHOL, as determined by electrophoretic mobility measurements. These results and the ethidium bromide fluorescence intercalation assays confirmed that more DOTAP: F7-CHOL liposomes are needed to compact the same amount of DNA as DOTAP: CHOL. The phase diagrams of aggregation and re-entrant condensation phenomena obtained by phenomenological theory support this conclusion and also establish that the liposome-DNA binding is stronger in the case of the DOTAP:F7-CHOL-DNA system. The stability rates of both liposomes in the presence of DNA and their transfection efficiencies were similar, which support the application of heptafluorocholesterol as a helper lipid in cationic liposomes for DNA delivery.
Subject:Health sciences, Technological sciences, Materials engineering Ciências da Saúde, Ciências Tecnológicas, Engenharia dos materiais
Country:Portugal
Document type:journal article
Access type:Restricted
Associated institution:Repositório Aberto da Universidade do Porto
Language:English
Origin:Repositório Aberto da Universidade do Porto

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